Development of Dimethylsulfonium Probes for Broad Profiling of Methyllysine Reader Proteins.

ABSTRACT: Lysine methylation is a crucial post-translational modification regulating various cellular processes. Reader proteins recognize specific methylated proteins as key mediators for the biological function of lysine methylation. Due to a correlation between reader activity and cell signaling of disorders, readers serve as attractive therapeutic targets. Despite proteomic advances identifying thousands of methylation sites, far fewer methyllysine readers have been characterized. Current research relies exclusively on site-specific probes, which are restricted to individual sites but incapable of global profiling. Here, an oligoglycine-based dimethylsulfonium peptide is reported as a general probe that is capable of binding and crosslinking to readers without bias toward particular sites or dom

SUBMITTER: Yang J 

PROVIDER: S-EPMC12904088 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature