Gelatin Methacrylate Macroporous Cell Scaffold Fabrication via One-Pot Aqueous Two-Phase Separation.

ABSTRACT: Microgels are increasingly recognized as versatile building blocks for granular cell scaffolds, offering advantages over bulk hydrogels for a variety of biomedical applications. While existing methods for scaffold fabrication often require multistep processes involving separate microgel formation and assembly, here we introduce a streamlined, one-pot approach that achieves microgel formation and scaffold assembly in minutes. This developed method is robust, reproducible, user-friendly, and requires no specialized equipment, making it broadly accessible. Specifically, aqueous two-phase separation (ATPS) was utilized to form ~2 μm gelatin methacrylate (GelMA) microgels in sodium sulfate salt solution, which rapidly "clicked" to form macroporous scaffolds under UV light. Various parameters we

SUBMITTER: Ferchichi E 

PROVIDER: S-EPMC12904336 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature