The three-dimensional landscape of tumor-associated macrophages in reactive and neoplastic human lymph nodes.

ABSTRACT: Macrophages play a crucial role in the homeostasis of lymph nodes. Their phenotypes and functions are dictated by the molecular composition of the microenvironment. The presence of tumor cells can influence the microenvironment, leading to changes in the cellular functions of tumor-associated macrophages (TAMs). Cellular alterations often correlate with histomorphometric and distributional changes. This study aims to characterize these pathomic modifications resulting from tumor cell infiltration by comparing them to reactive conditions. We assessed CD68+ and CD163+ TAMs in 160 three-dimensional (3D) images of human lymphoid tissue sections derived from 82 cases comprising six different diagnoses. To investigate TAM profiles, we employed a hybrid approach involving computer vision and grap

SUBMITTER: Vladisavljevic A 

PROVIDER: S-EPMC12904395 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature