Longitudinal investigation of prostate tumor spheroid proliferation with dynamic line-field optical coherence tomography.

ABSTRACT: Recently, it has become widely recognized that culturing cancer cells in vitro in small, 3D aggregates known as tumor spheroids provides a more physiologically relevant model of in vivo tumor behavior compared to 2D monolayer cultures. Dynamic optical coherence tomography (dOCT) is a non-invasive imaging modality that, by analyzing temporal fluctuations in the light scattered from biological tissue, does not require exogenous contrast agents to visualize and quantify cellular activity within 3D cell cultures. However, recent volumetric dOCT studies have encountered challenges due to low acquisition speeds. In this study, we present morphological and dynamic analyses of prostate tumor spheroid growth over a two-week longitudinal period, utilizing a line-field dOCT platform. Vo

SUBMITTER: Swanson S 

PROVIDER: S-EPMC12904543 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature