Enhancing the efficiency of achieving optical transparency in live animals using absorbing molecules.

ABSTRACT:

Significance

In vivo optical imaging is crucial for studying disease mechanisms but is limited by light scattering and poor penetration in biological tissues. While tissue-clearing reagents (hydrophilic/hydrophobic) and bioluminescent probes improve imaging, achieving effective optical transparency in live tissues remains a challenge. This study builds on recent work using absorbing dyes (tartrazine and 4-aminoantipyrine) to enhance in vivo tissue clearing, aiming to optimize efficacy and biosafety.

Aim

We aimed to develop a mixed solution of tartrazine and 4-aminoantipyrine (4-AA) that improves optical transparency, accelerates clearing, and reduces toxicity compared to individual dyes, enabling safer and more efficient deep-tissue imaging in live animals.

SUBMITTER: Sun T 

PROVIDER: S-EPMC12904770 | biostudies-literature | 2026 May

REPOSITORIES: biostudies-literature