Wood Hemicellulose-Based Spray-Dried Microencapsulation of a Lytic Bacteriophage Preserves Phage Viability and Improves Control of the Bacterial Wilt Pathogen Ralstonia solanacearum.

ABSTRACT: Ralstonia solanacearum (RS) is a soil-borne phytopathogen responsible for bacterial wilt disease on a wide range of crops worldwide. Bacteriophage biocontrol is a promising sustainable RS management method. However, more work is needed to design methods to store, ship and apply phage that are effective, scalable and environmentally friendly. Here, we investigate the use of wood hemicellulose excipients-glucuronoxylans (GX) and galactoglucomannans (GGM) - to encapsulate phage PYO4, which can infect the pandemic RS strain UW551. Yield and preservation efficiencies of GX and GGM were compared to the conventional excipient maltodextrin (MD). Encapsulation via spray drying was carried out at two inlet/outlet temperatures, and the resulting powders were stored at room temperature or at 4°C. Phag

SUBMITTER: Negroni AM 

PROVIDER: S-EPMC12904778 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature