Systematic scRNA-seq screens profile neural organoid response to morphogens.

ABSTRACT: Morphogens direct neuroepithelial fates toward discrete regional identities in vivo. Neural organoids provide models for studying neural regionalization through morphogen exposure; however, we lack a comprehensive survey of how the developing human neuroepithelium responds to morphogen cues. Here we produce a detailed survey of morphogen-induced effects on the regional specification of human neural organoids using multiplexed single-cell transcriptomic screens. We find that the timing, concentration and combination of morphogens strongly influence organoid cell-type and regional composition, and that cell line and neural induction method impact the response to a given morphogen condition. We apply concentration gradients in microfluidic chips or increasing static concentrations in multi-we

SUBMITTER: Sanchis-Calleja F 

PROVIDER: S-EPMC12904787 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature