Detection of highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b virus in cull dairy cows with underlying respiratory and systemic disease.

ABSTRACT: Highly pathogenic avian influenza (HPAI) A(H5N1) clade 2.3.4.4b virus was identified in 4 cull dairy cows condemned by the U.S. Department of Agriculture because of pneumonia with accompanying systemic changes. Histologic findings were bronchopneumonia in 3 cows and embolic pneumonia and nephritis in 1 cow. In addition to detection of HPAI A(H5N1) virus by reverse-transcription real-time PCR in various formalin-fixed, paraffin-embedded tissues, influenza A virus was detected by immunohistochemistry and in situ hybridization in the pulmonary respiratory epithelium of 2 of the cows with bronchopneumonia and in renal medullary tubules of the cow with nephritis. A PCR panel screening for common bovine respiratory pathogens in the cows with bronchopneumonia revealed variable coinfections with <

SUBMITTER: Righter DJ 

PROVIDER: S-EPMC12904801 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature