Hydrogen sulfide promotes wheat immunity against stripe rust through TaATG6c persulfidation.

ABSTRACT: Hydrogen sulfide (H₂S) is a key gaseous signaling molecule involved in plant growth and stress responses, yet its role in wheat resistance to stripe rust remains poorly understood. Here, we show that exogenous H₂S enhances resistance of wheat (Triticum aestivum L.) to Puccinia striiformis f. sp. tritici (Pst), the causative agent of stripe rust. Comparative persulfidation proteomics identified the autophagy-related protein TaATG6c as a Pst-responsive H₂S target. Site-specific mass spectrometry and a modified biotin-switch assay demonstrated that Cys177 and Cys180 of TaATG6c undergo H₂S-induced persulfidation. Structural modeling based on AlphaFold predicted that these two site mutations reduced the binding activity of ATG6c to ATG14. Functional characterization using

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12905053 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature