Central amygdala single-nucleus atlas reveals chromatin and gene transcription dynamics in human alcohol use disorder.

ABSTRACT: Regulation of gene expression is a highly coordinated process in both the healthy and pathological brain with unique patterns across a multitude of cell types. Here we present a multi-omic single nucleus study of ~175,000 nuclei from 50 donors with alcohol use disorder (AUD) and control donors without AUD, profiling cell type specific gene expression and chromatin accessibility in the human central amygdala. We identify all major CNS cell types and neuronal subtypes and find inhibitory neurons are particularly affected by AUD. We find high numbers of differentially expressed genes (DEGs) including GABRA2, GRM8, and NCAM1 and show significant enrichment for AUD risk genes within these DEGs. We identified 51,431 cell type-specific, disease associated candidate cis-regulatory elements includi

SUBMITTER: Lee CY 

PROVIDER: S-EPMC12905162 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature