Development and preliminary evaluation of real-time PCR assays for six lactic acid bacteria.

ABSTRACT: This study developed new real-time PCR assays for six lactic acid bacteria (LAB): Ligilactobacillus agilis, Limosilactobacillus fermentum, Lactobacillus johnsonii, Ligilactobacillus salivarius, Pediococcus pentosaceus, and Weissella cibaria for their application in the food industry. For each target bacterium, a novel primer/probe set was designed in a conserved and species-specific genomic region, with proper length (13 ~ 30nt), Tm (probes: 68 ~ 70℃; primers: 58 ~ 60℃), GC content (30 ~ 80%), and amplicon length (50 ~ 150 bp). The Gibbs free energy (ΔG, an indicator for stability of secondary structures) of potential hairpins, self-dimers, and cross-dimers of the primers and probes adhered largely to the recommended values with minor deviations. BLAST analysis verified the conservation an

SUBMITTER: Li SJ 

PROVIDER: S-EPMC12905260 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature