Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system.

ABSTRACT: Host expansion facilitates tephritid flies to expand their ranges. Unraveling the mechanisms of host expansion will help to efficiently control these pests. Our previous works showed mitochondrial complex I genes Ndufs1, Ndufs3, and Ndufa7 being upregulated during host expansion of Bactrocera tau (Walker), one of the highly hazardous species of tephritids. However, their roles in the host expansion of B. tau remain unknown. Here, using clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas9) editing system for the first time, a stable homozygous Ndufa7 strain (Btndufa7^-/-), heterozygous Ndufs1 (Btndufs1^+/-), and Ndufs3 strains (Btndufs3^+/-) were obtained from F3 generation of B. tau, after gene knockout. Reduc

SUBMITTER: Shi W 

PROVIDER: S-EPMC12905475 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature