Rapid bone formation and remodeling via vascular infiltration of a hydroxyapatite/collagen nanocomposite beneath the calvarial periosteum.

ABSTRACT: The development of biomaterials that actively promote bone regeneration remains a major challenge in regenerative medicine and dentistry. This study investigated the time-dependent osteogenic potential of an organic-inorganic composite bone filler (hydroxyapatite/collagen bone-like nanocomposite, HAp/Col) using a rat calvarial model, assessed by immunohistochemistry and bone histomorphometry. By day 3, vascular endothelial growth factor receptor 2 (VEGFR2)-positive cells were detected on the HAp/Col surface, indicating early vascular invasion. By day 5, vascular infiltration had extended into the scaffold, where double staining with tartrate resistant acid phosphatase (TRAP)/ alkaline phosphatase (ALP) revealed osteoclast-mediated material resorption and osteoblast-mediated bone matrix dep

SUBMITTER: Arai H 

PROVIDER: S-EPMC12905741 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature