<i>De novo</i> biosynthesis of eriocitrin in <i>Saccharomyces cerevisiae</i> through deep learning-guided enzyme screening and systematic metabolic engineering.

ABSTRACT: Eriocitrin, a flavanone-7-O-disaccharide known for its antioxidant and anti-inflammatory properties, holds considerable promise for use in functional foods and pharmaceuticals. However, its large-scale production is constrained by the limitations of conventional plant extraction, including low abundance of active compounds and seasonal variability. Here, we established a de novo biosynthetic pathway for eriocitrin from glucose in engineered Saccharomyces cerevisiae through combinatorial metabolic engineering. First, to verify the feasibility of glycosylation in the eriocitrin synthesis process, two highly efficient flavonoid-7-O-glucosyltransferases were identified using an integrated bioinformatics and deep learning approach. Subsequently, through the reconstru

SUBMITTER: Lu Q 

PROVIDER: S-EPMC12905779 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature