Dataset Information

Urinary miR-191-5p levels are significantly reduced after radical prostatectomy in patients with prostate cancer.

ABSTRACT:

SUBMITTER: De Palma FDE

PROVIDER: S-EPMC12905937 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Urinary miR-191-5p levels are significantly reduced after radical prostatectomy in patients with prostate cancer.

De Palma Fatima Domenica Elisa FDE Carbonnier Vincent V Cernera Gustavo G Gelzo Monica M Nardelli Carmela C Pandolfo Savio Domenico SD Aveta Achille A Perdonà Sisto S Imbimbo Ciro C Castaldo Giuseppe G

Cancer cell international 20260120 1

BACKGROUND: MicroRNAs (miRNA) detection in urine samples may be a scarce invasive approach for diagnosis and monitoring prostate cancer (PCa). The role of miR-191-5p in prostate oncogenesis, as well as its function as a potential diagnostic and prognostic biomarker has been described in many cancers. However, its role as a diagnostic non-invasive indicator in PCa is still under investigated. METHODS: We performed an extracellular vesicle (EV)-based miRNA-sequencing of urine samples (n = 12/group ...[more]

PMID: 41559725

Similar Datasets

Project description:Purpose: Available tools for prostate cancer (PC) diagnosis and prognosis are suboptimal and novel biomarkers are urgently needed. Here, we investigated the regulation and biomarker potential of the GABRE~miR-452~miR-224 genomic locus. Experimental design: GABRE/miR-452/miR-224 transcriptional expression was quantified in 80 non-malignant and 281 PC tissue samples. GABRE promoter methylation was determined by methylation-specific qPCR (MethyLight) in 35 non-malignant, 293 PC (radical prostatectomy (RP) cohort 1) and 198 PC tissue samples (RP cohort 2). Diagnostic/prognostic biomarker potential of GABRE methylation was evaluated by ROC, Kaplan-Meier, uni- and multivariate Cox regression analyses. Functional roles of miR-224 and miR-452 were investigated in PC3 and DU145 cells by viability, migration, and invasion assays and gene-set enrichment analysis (GSEA) of post-transfection transcriptional profiling data. Results: GABRE~miR-452~miR-224 was significantly downregulated in PC compared to non-malignant prostate tissue and had highly cancer-specific aberrant promoter hypermethylation (AUC=0.98). Functional studies and GSEA suggested that miR-224 and miR-452 inhibit proliferation, migration, and invasion of PC3 and DU145 cells by direct/indirect regulation of pathways related to the cell cycle and cellular motility. Finally, in uni- and multivariate analyses, high GABRE promoter methylation was significantly associated with biochemical recurrence in RP cohort 1, which was successfully validated in RP cohort 2. Conclusion: The GABRE~miR-452~miR-224 locus is downregulated and hypermethylated in PC and is a new promising epigenetic candidate biomarker for PC diagnosis and prognosis. Tumor suppressive functions of the intronic miR-224 and miR-452 were demonstrated in two PC cell lines, suggesting that epigenetic silencing of GABRE~miR-452~miR-224 may be selected for in PC. Affymetrix GeneChip Human Gene 1.0 ST Arrays were used for whole-genome transcriptional profiling of DU145 and PC3 cells at 48 hours post-transfection with either miR-224, miR-452, or scrambled miRNA mimics, or untransfected. All experiments were performed in duplicate. Transcript expression levels were determined after RMA16 normalization in GeneSpringGX 11.0 software (Agilent). PC3 and DU145 arrays were normalized separately. DU145 48 t Scr2a were excluded from the study because of bad performance of this microarray.

Dataset Information

Urinary miR-191-5p levels are significantly reduced after radical prostatectomy in patients with prostate cancer.

Publications

Urinary miR-191-5p levels are significantly reduced after radical prostatectomy in patients with prostate cancer.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets