Molecular prevalence of <i>Ehrlichia canis</i> in dogs examined at the Hospital de Clinicas Veterinarias of Universidade Federal de Pelotas, Southern Rio Grande do Sul, Brazil.

ABSTRACT: Ehrlichia canis is a pathogen that causes a multisystemic disease in dogs of all ages and sexes and belongs to a genus with zoonotic potential. This study aimed to determine the molecular prevalence of E. canis in a population of dogs with no clinical suspicion of ehrlichiosis, examined at the Hospital de Clínicas Veterinária, Universidade Federal de Pelotas (HCV, UFPel). Blood samples from 95 dogs were analyzed at the Veterinary Molecular Biology Laboratory (LabMol-Vet) using nested PCR (nPCR) targeting a 16S rDNA fragment. Amplified products were analyzed by electrophoresis on 1.5% agarose gel and visualized under UV light, revealing a molecular prevalence of 16.84% (16/95). Two PCR products were sequenced for phylogenetic analysis, providing complementary confirmation. Thi

SUBMITTER: Linares DFB 

PROVIDER: S-EPMC12906024 | biostudies-literature | 2026 May

REPOSITORIES: biostudies-literature