EGR1/miR-4306/MAT2A axis aggravates pyroptosis and extracellular matrix degradation of nucleus pulposus cells in intervertebral disc degeneration.

ABSTRACT:

Background

Intervertebral disc degeneration (IVDD) acts as the prerequisite and pathological basis for a series of spinal degenerative diseases, and it remains one of the most harmful and difficult-to-treat conditions in this category. However, the exact pathogenesis of IVDD has not been fully elucidated.

Methods

Comprehensive analysis of data mining, bioinformatics and real-time quantitative PCR was used to pinpoint the key transcription factors participated in the progression of IVDD. The role of early growth response factor 1 (EGR1) in IVDD was determined through a series of loss- and gain-of-function experiments in vitro and in vivo. Mechanistically, bioinformatics, chromatin immunoprecipitation, and dual-luciferase reporter assays were applied to illustrate the interact

SUBMITTER: Qiu S 

PROVIDER: S-EPMC12906533 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature