<i>In vitro</i> polyploid induction in <i>Allium grayi</i> Regel using colchicine.

ABSTRACT: Allium grayi Regel is a seasonally limited small perennial herb of the subfamily Allioideae, and is endemic to Matsu, Taiwan. The species possesses nutritious, ornamental value, and biological pharmacological activity. This study evaluated for the first time to induce polyploidy using the dissected bulb of A. grayi as explants in vitro, with the expectation of increasing the bulb size. Sterile bulbs were divided into four equal parts and pre-cultured under different durations before being soaked in a one g/L colchicine solution for 12 or 24 hours. Survival, regeneration, variation, and tetraploid induction rates were recorded, while ploidy levels were determined by flow cytometry and stomatal traits were measured microscopically. The results showed that pre-cult

SUBMITTER: Chen TS 

PROVIDER: S-EPMC12906704 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature