The effects of alcohol dependence on the CSF proteome in mice: Evidence for blood-brain barrier dysfunction and neuroinflammation.

ABSTRACT: Alcohol use disorder (AUD) represents a significant neurological health burden, yet the biological mechanisms underlying alcohol-induced brain pathology remain incompletely understood. Moreover, the molecular underpinnings of the transition from alcohol exposure to alcohol dependence are not well-characterized. We used mass spectrometry (MS)-based proteomics in a preliminary discovery study to compare cerebrospinal fluid (CSF) of alcohol-exposed Non-dependent (Non-dep) versus alcohol-dependent (Dep) mice that underwent the chronic intermittent ethanol (alcohol) - two-bottle choice (CIE-2 BCE) procedure and systemic anti-IL-6 Receptor antibody administration (n = 9; female = 5, male = 4). CSF samples from individual mice were processed for proteomic analysis and digested with trypsin overni

SUBMITTER: Turner NP 

PROVIDER: S-EPMC12906712 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature