Rosmarinic Acid Synthase 1 Phosphorylation by SmMAPK3 Is Required for Salicylic Acid-Induced Salvianolic Acid Accumulation in Salvia miltiorrhiza Hairy Roots.

ABSTRACT: Salvianolic acid is the main active component of Salvia miltiorrhiza and holds significant value in the clinical treatment of myocardial ischemia and hypoxia. Previous studies have shown that salicylic acid (SA) can significantly promote the accumulation of salvianolic acid, but its molecular mechanism remains incompletely understood. To reveal the molecular mechanism by which SA regulates salvianolic acid, this study treated transgenic hairy roots overexpressing mitogen-activated protein kinase 3 (SmMAPK3) with SA and demonstrated that SmMAPK3 is essential for SA-induced salvianolic acid biosynthesis, with this function relying on the kinase activity of SmMAPK3. Through yeast two-hybrid screening, Rosmarinic Acid Synthase 1 (SmRAS1) was identified as a direct interacting protein of SmMAPK

SUBMITTER: Yin X 

PROVIDER: S-EPMC12906818 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature