Fut8-Mediated Core Fucosylation of Toll-Like Receptor 4 Exacerbates Periodontitis Via Hyperactivation of NF-κB Signalling.

ABSTRACT:

Background

Chronic inflammation in periodontitis is linked to aberrant glycosylation, yet the molecular mechanisms and therapeutic potential of the core fucosyltransferase Fut8 remain undefined.

Methods

Gingival samples from healthy individuals and periodontitis patients were collected. Glycosylation levels were assessed using histological and molecular biological techniques. A mouse periodontitis model was established and injected with the core fucosylation inhibitor 2FF. Integrated transcriptomic and single-cell sequencing data were analysed to screen for key molecules. An in vitro human gingival fibroblast model was utilized. Gene silencing, coimmunoprecipitation, and pathway analysis were employed to elucidate the Fut8-Toll-like receptor 4 (TLR4) regulatory mechanism.

SUBMITTER: Lin J 

PROVIDER: S-EPMC12907083 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature