Complex cooperativity in DNA origami revealed via design-dependent defectivity.

ABSTRACT: DNA origami has become a ubiquitous platform because it enables straightforward design of nanostructures that self-assemble with high yield. The interactions between the cooperative effects involved in its assembly are currently not well understood. Fortunately, the nearly infinite number of choices available to the origami designer provides a rich environment in which to explore cooperativity. The DNA domains comprising origami have predictable energetics, and the sources of cooperativity are conceptually straightforward, and the difficulty in predicting assembly comes from their large number of cooperative interactions. We are able to probe cooperativity by using design variations and measuring their effect on assembly yield. We employ an accelerated assembly protocol that increases the

SUBMITTER: Majikes JM 

PROVIDER: S-EPMC12907558 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature