Proline promotes prolyl 4-hydroxylase subunit alpha 1 via transforming growth factor beta 1 to increase the synthesis and deposition of collagen in grass carp (<i>Ctenopharyngodon idellus</i>) muscle fibroblasts: Insights from integrated bioinformatics and functional analysis.

ABSTRACT: The present study aimed to clarify the molecular characteristics of prolyl 4-hydroxylase subunit alpha 1 (p4ha1) and elucidate its regulatory role in collagen synthesis in grass carp (Ctenopharyngodon idellus) muscle. To this end, the full-length coding DNA sequence (CDS) of muscle p4ha1 was first cloned and subjected to bioinformatics analysis, with sequence characterization specifically performed via prediction of open reading frame (ORF) and corresponding amino acid sequence, analysis of physical and chemical property, identification of conserved domain, alignment of amino acid sequence, and construction of the phylogenetic tree. The results revealed that the p4ha1 CDS is 1623 bp, encoding 540 amino acids, with a molecular weight of approximately 61.78 kDa an

SUBMITTER: Lin Y 

PROVIDER: S-EPMC12907842 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature