Functional CRISPR-Cas9 knockout screening of the genetic determinants of human fibroblast migration propensity.

ABSTRACT: Directional cell migration plays a central role in a wide range of physiological and pathological conditions, such as embryonic development or tumor metastasis. Steps involved in cell migration include cell polarization, formation of membrane protrusions at the cell front side and adhesion disassembly at the rear side, and a general cytoskeletal rearrangement. Overall, it is a complex phenomenon at the interface between mechanical forces and biochemical signaling, with cell-specific and context-specific molecular events acting in the process. Here, we focus on human fibroblast migration induced by a biochemical gradient with an approach that connects the identification of molecular players with the actual mechanical function. We show how to screen for genes and miRNAs involved in migration

SUBMITTER: Mazzei A 

PROVIDER: S-EPMC12908101 | biostudies-literature | 2026 Jan-Feb

REPOSITORIES: biostudies-literature