Scale-up of a monoclonal antibody CHO fed-batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen.

ABSTRACT: Key hydrodynamic-related parameters such as volumetric power input (P/V), impeller configuration, aeration strategy, and maximum gas sparge rate, as well as an appropriate feeding strategy, must be carefully selected to improve production yields in bioreactor. In this study, the feeding regimen was found to have an important impact on cell growth and productivity of a cumate-inducible CHO fed-batch cell culture. A low-volume feeding regimen avoided a rapid increase in osmolality, allowing for prolonged cell viability and a 33% increase in volumetric titer compared to the high-volume feeding regimen. Both sparged air and oxygen were used for dissolved oxygen (DO) control, utilizing three levels of airflow rates. An optimum airflow rate of 0.0031 vvm was found to improve cell growth, longevi

SUBMITTER: Lemire L 

PROVIDER: S-EPMC12908111 | biostudies-literature | 2026 Jan-Feb

REPOSITORIES: biostudies-literature