Metabolic engineering for improved heterologous pinene production in the chloroplast of Phaeodactylum tricornutum.

ABSTRACT:

Background

The marine diatom Phaeodactylum tricornutum is a promising platform for the sustainable production of terpenoids. This is due to its robust photosynthetic growth and natural accumulation of terpenoids, mainly including photosynthetic pigments. P. tricornutum harbors the methyl-erythritol-phosphate (MEP) pathway in the chloroplast, a dedicated route used for the production of terpenoid-like photosynthetic pigments. Despite its natural predisposition for terpenoid production in the chloroplast, previously reported titers of heterologously produced terpenoids in P. tricornutum are relatively low.

Results

In this study, we used a metabolic engineering strategy to enhance the production of the terpenoid pinene by increasing the production of their precursors. We episom

SUBMITTER: Trevisan N 

PROVIDER: S-EPMC12908318 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature