Gene discovery and expression analysis of the B cell receptor repertoire in the domestic ferret model.

ABSTRACT: The domestic ferret is the preferred model organism for the study of influenza A infection and responses to vaccination, because its respiratory tract architecture and sialic acid receptor type and distribution are similar to those in humans. Despite this, the ferret has remained underutilized in antibody-omics research, which is increasingly critical to inform vaccine design. The molecular analysis of antibody responses is predicated on precise knowledge of the germline V(D)J segments, and is an active area of research for this species. To define a reference immunoglobulin repertoire for the ferret, we used a curated set of V(D)J genes from human and closely related carnivores to BLAST the ferret genome. Non-overlapping BLAST hits were annotated and vetted for recombination signal sequenc

SUBMITTER: Hebert LS 

PROVIDER: S-EPMC12908631 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature