Single molecule counting detects low-copy glycine receptors in hippocampal and striatal synapses.

ABSTRACT: Glycine receptors (GlyRs) are heteropentameric chloride channels that mediate fast inhibitory neurotransmission in the brainstem and spinal cord, where they regulate motor and sensory processes. GlyRs are clustered in the postsynaptic membrane by strong interactions of the β subunit with the scaffold protein gephyrin. Even though Glrb mRNA is highly expressed throughout the brain, the existence of synaptic GlyRs remains controversial as there is little conclusive evidence using conventional fluorescence microscopy and electrophysiological recordings. Here, we exploit the high sensitivity and spatial resolution of single molecule localisation microscopy (SMLM) to investigate the presence of GlyRs at inhibitory synapses in the brain, focusing on several areas of the telencephalon. Mak

SUBMITTER: Camuso S 

PROVIDER: S-EPMC12908935 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature