Comparison of the phenolic and antioxidant potential of five European herbal remedies by effect-directed analysis using offline two-dimensional liquid chromatography-high resolution mass spectrometry.

ABSTRACT: Herbal remedies contain various phenolic compounds. However, it remains difficult to identify the most important bioactive components and compare their effectiveness in different plant species. In this study, effect-directed analysis has been applied to five European medicinal plants. Angelica archangelica, Angelica sylvestris, Agrimonia eupatoria, Sambucus ebulus, and Sambucus nigra have been analyzed to unravel and compare their phenolic profiles and antioxidant potential. Plant extracts obtained by a sustainable microwave-assisted extraction method were fractionated using semi-preparative liquid chromatography to yield continuous fractions, and a miniaturized ABTS radical scavenging assay of the fractions was used to screen for antioxidant activity. Highly active fractions were selected

SUBMITTER: Haßler M 

PROVIDER: S-EPMC12909492 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature