Establishment of a Novel Human Endometrial Organoid.

ABSTRACT:

Purpose

Human implantation is difficult to study in vivo because of ethical constraints, making the development of in vitro models essential for mechanistic investigations. Here, we applied the technique previously established in mice to develop a novel human endometrial organoid.

Methods

Human endometrial tissues were digested and cultured as adherent monolayers without extracellular matrix (ECM). After 10 days, self-organized aggregates appeared and were transferred to low-attachment plates for three-dimensional culture. To assess hormonal responsiveness, organoids were treated with medroxyprogesterone acetate and cyclic adenosine monophosphate for 4 days.

Results

The resulting organoids consisted of EpCAM-positive epithelial cells forming an outer layer and PDGFRα-

SUBMITTER: Yoneda T 

PROVIDER: S-EPMC12909605 | biostudies-literature | 2026 Jan-Dec

REPOSITORIES: biostudies-literature