MTORC1-dependent suppression of autophagic activity in somatic cell nuclear transfer mouse embryos.

ABSTRACT:

In brief

The non-genomic factors responsible for developmental arrest in SCNT embryos remain poorly understood. Using live-cell fluorescence imaging, we revealed that autophagic activity is impaired in preimplantation SCNT embryos, possibly due to ectopic activation of the mTORC1 signaling pathway, providing new insights into cytoplasmic barriers to cloning efficiency.

Abstract

Activation of autophagy after fertilization is essential for mammalian embryonic development, as it supplies embryos with nutrients and energy. Somatic cell nuclear transfer (SCNT) embryos frequently exhibit developmental arrest, largely because of incomplete genomic reprogramming; however, the role of non-genomic factors remains unclear. Here, we investigated autophagy dynamics in mouse SCNT embryos

SUBMITTER: Tatebe T 

PROVIDER: S-EPMC12910570 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature