METTL14 knockdown mitigates HG-induced HK-2 cell apoptosis, inflammation, and ferroptosis by promoting SLC40A1.

ABSTRACT:

Background

Diabetic nephropathy (DN) represents a leading cause of chronic kidney diseases worldwide. While solute carrier family 40 member 1 (SLC40A1) is involved in the regulation of iron homeostasis and iron transport in diabetic kidney disease, its specific role and underlying mechanism in regulating ferroptosis during DN progression remain elusive.

Methods

Protein and mRNA expression levels were detected using western blot and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Cell viability and apoptosis were measured using the cell counting kit-8 (CCK8) and flow cytometry assays. The tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), ferrous iron (Fe²⁺), reactive oxygen species (ROS), glutathione (GSH), and malondialdehyde (MD

SUBMITTER: Jin G 

PROVIDER: S-EPMC12910788 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature