Consensus-based computational mapping of NS1 antigenic determinants in dengue and Zika viruses to improve diagnostic specificity.

ABSTRACT:

Background

Cross-reactivity between samples from phylogenetically related organisms remains a significant challenge in antigen detection by lateral flow assays. Recent advancements in bioinformatics tools have significantly enhanced our understanding of the complex interactions between antigenic proteins and antibodies, paving the way for the development of more precise and effective solutions to address these challenges.

Methods

In this study, we used two common modeling tools, SWISS-MODEL and AlphaFold (2 and 3), to construct three-dimensional (3D) models of the nonstructural 1 (NS1) protein from dengue (DENV) and Zika viruses (ZIKV) based on their protein sequences. Both linear and 3D structures of the proteins were used to predict antigenicity (Jameson-Wolf and Kolaskar-

SUBMITTER: Valotto RS 

PROVIDER: S-EPMC12910838 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature