DNMT1 deficiency promotes pulmonary vascular remodeling in pulmonary hypertension through epigenetic upregulation of BMP7.

ABSTRACT:

Background

Pulmonary hypertension (PH) is a progressive cardiopulmonary disorder marked by pathological vascular remodeling, predominantly driven by the hyperproliferation of pulmonary artery smooth muscle cells (PASMCs). Although bone morphogenetic protein 7 (BMP7) has been implicated in PASMCs dysregulation, the precise upstream mechanisms governing its expression in PH remain elusive.

Methods

In vitro and in vivo models of hypoxia-induced PH were employed. The mRNA and protein levels of the different molecules were quantified using qRT-PCR and Western blot. The role of BMP7 in PASMCs proliferation, cell cycle progression and apoptosis were assessed using Cell Counting Kit-8 (CCK-8) assays, Western blotting, immunofluorescence, and flow cytometry. Moreover, the epigenetic

SUBMITTER: Wang YB 

PROVIDER: S-EPMC12910850 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature