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Linking metagenomic insight to cultivable microbes: isolation of a vitamin B<sub>12</sub>-producing Sphingomonad from Indonesian tempeh.

ABSTRACT:

SUBMITTER: Wijaya SC 

PROVIDER: S-EPMC12911007 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature

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Linking metagenomic insight to cultivable microbes: isolation of a vitamin B&lt;sub&gt;12&lt;/sub&gt;-producing Sphingomonad from Indonesian tempeh.

Wijaya Sena Chandra SC   Richi Mario M   Waturangi Diana Elizabeth DE   Yulandi Adi A  

BMC microbiology 20260122 1

BACKGROUND: Tempeh, a famous traditional Indonesian fermented soybean product, reportedly contains vitamin B12. Although Enterobacteriaceae have been previously implicated in vitamin B12 production in tempeh, the function of Sphingomonadaceae, which is abundant in some tempeh samples, remains unknown. This study aimed to identify and characterize vitamin B12-producing bacteria from Empang (EMP) tempeh, with a focus on the understudied Sphingomonad. RESULTS: Metagenomic analysis focusing on vitam  ...[more]

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