Population structure and zoonotic potential of Cryptosporidium parvum in Italy inferred using a multi-locus sequence typing scheme.

ABSTRACT:

Background

The genetic variability of a large collection of European samples of the zoonotic pathogen Cryptosporidium parvum has been recently explored on the basis of a novel multi-locus sequence typing (MLST) scheme. In this work, we assessed the usefulness of this scheme to type C. parvum samples from Italy, a country where this pathogen is widespread and associated with human infections.

Methods

Polymerase chain reaction (PCR) and sequencing for the eight markers of the MLST scheme were performed on 31 human- and 21 animal-derived C. parvum samples. MLST data from 27 samples of animal origin previously sequenced at the genome level were also included. Sequence data for the glycoprotein 60 (gp60) gene were also generated. Phylogenetic and cluster analyses were conducted.<

SUBMITTER: Ascierto M 

PROVIDER: S-EPMC12911039 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature