Comparative evaluation of nine lateral flow assays for FIV antibody detection using an in-house ELISA as a reference method.

ABSTRACT: Feline immunodeficiency virus (FIV) induces immunosuppression in affected cats, increasing susceptibility to chronic and secondary infections. Rapid and accurate detection of FIV-specific antibodies is essential for effective clinical management and epidemiological monitoring. This study conducted a comparative evaluation of nine commercially available lateral flow assays (LFAs) for detecting FIV antibodies in whole blood, serum, or plasma, using a newly developed in-house enzyme-linked immunosorbent assay (ELISA) as a reference method. All tested LFAs demonstrated 100% specificity. While Vet Expert new and VetFor achieved 100% across all metrics indicating the best performance, formal statistical comparison did not reveal significant differences between the evaluated kits. Overall, the re

SUBMITTER: Laska-Modzelewska A 

PROVIDER: S-EPMC12911111 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature