MiR-596 as a novel prognostic biomarker and tumor suppressor in breast cancer through targeting EIF5AL1.

ABSTRACT:

Background

In terms of global incidence and mortality, breast cancer continues to surpass all other cancers affecting women.

Methods

To explore the role of miR-596, qRT-PCR was applied to measure its expression in tissue and cell samples from 137 enrolled subjects. The regulatory interaction between miR-596 and EIF5AL1 was verified by dual-luciferase reporter assays. CCK-8 and Transwell assays were utilized to respectively measure the proliferation, migration, and invasion capabilities of the two breast cancer cell lines, MCF-7 and MDA-MB-231.

Results

A significant downregulation of miR-596 was observed in breast cancer tissues and cell lines (all P < 0.001). Clinically, reduced miR-596 expression was associated with advanced TNM stage, lymph node metastasis, and infe

SUBMITTER: Huang R 

PROVIDER: S-EPMC12911248 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature