Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis.

ABSTRACT:

Objective

This study aimed to determine the role and mechanism underlying migration and invasion inhibitory protein (MIIP) modulation in M2 macrophages within the tumor microenvironment and the potential of targeting the MIIP- stimulator of interferon genes (STING) pathway in colorectal cancer (CRC) therapy.

Methods

MIIP expression was analyzed for associations with the STING pathway and M2 macrophage infiltration using public datasets and clinical CRC samples. CRC cells were genetically modified using lentiviral vectors to overexpress or silence MIIP and STING. The interactions of genetically modified CRC cells with macrophages were studied in co-culture systems. Techniques, including immunofluorescence staining, RT-qPCR, western blot, ELISA, flow cytometry, and Transwell m

SUBMITTER: Chen S 

PROVIDER: S-EPMC12911439 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature