Dynamic sampling from <i>ex vivo</i> adipose tissue using droplet-based microfluidics supports separate mechanisms for glycerol and fatty acid secretion.

ABSTRACT: Pathologies in adipose (fat) tissue function are linked with human diseases such as diabetes, obesity, metabolic syndrome, and cancer. Dynamic, rapid release of metabolites has been observed in adipocyte cells and tissue, yet higher temporal resolution is needed to adequately study this process. In this work, a microfluidic device with precise and regular valve-automated droplet sampling, termed a microfluidic analog-to-digital converter (μADC), was used to sample secretions from ∼0.75 mm diameter adipose explants from mice, and on-chip salt water electrodes were used to merge sampled droplets with reagent droplets from two different fluorometric coupled enzyme assays. By integrating sampling and assays on-chip, either glycerol or non-esterified fatty acids (NEFA), or both, were quantified

SUBMITTER: Moniruzzaman M 

PROVIDER: S-EPMC12911469 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature