Analysis of MHC class II-bound CyHV-2 peptides in <i>Carassius gibelio</i> using mass spectrometry.

ABSTRACT: Class II major histocompatibility complexes (MHC-II) are a highly polymorphic and multigenic family of molecules that present exogenous peptides to CD4⁺ helper T cells, thereby activating the host adaptive immune system. In this study, we systematically analyzed the genomic distribution and tissue-specific expression of MHC-II genes in Carassius gibelio. The Cagi-DDA/DFA molecule was found to be highly expressed in the spleen and head kidney, moderately expressed in the intestine, gills, and trunk kidney, and expressed at low levels in the liver and brain. A polyclonal antibody was generated against the most prevalent Cagi-DDA/DFA allele in the population. Using immunopeptidomics, we identified viral peptides bound to Cagi-DDA/DFA molecules in the hea

SUBMITTER: Xu C 

PROVIDER: S-EPMC12911862 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature