Deciphering immune features and cellular heterogeneity in PRRSV infection via single-cell RNA sequencing.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV), a major immunosuppressive pathogen, inflicts substantial economic losses on the global swine industry. Despite extensive research into PRRSV pathogenesis, the mechanisms by which PRRSV induces immune dysfunction in vivo remain incompletely understood. Here, we performed single-cell RNA sequencing on cells isolated from the lung of PRRSV-infected piglets, generating transcriptomic profiles for 46,922 single cells encompassing 15 major cell types. We observed a significant reduction in the number of macrophages in lung tissues, which was primarily attributed to the extensive apoptosis of macrophages induced by PRRSV infection. PRRSV infection triggered aberrant differentiation of macrophage, and the SPP1^high macro

SUBMITTER: Li J 

PROVIDER: S-EPMC12911900 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature