Optimizing adipogenic cocktail composition to enhance beige adipogenesis and evaluate thermogenic potential in primary mouse subcutaneous fat cell cultures.

ABSTRACT:

Background

Subcutaneous white adipose tissue (WAT) is crucial for systemic metabolic homeostasis, with beige adipocytes in this depot contributing to energy metabolism through inducible thermogenesis. Differentiated adipocyte cultures derived from mouse inguinal WAT are a widely used system to study beige adipose biology and identify therapeutic targets, as they retain the genetic and epigenetic traits of native adipocytes while providing experimental flexibility. However, variability during the adipocyte induction and differentiation poses a challenge, impacting beige adipogenesis and experimental outcomes.

Methods

This study conducted an unbiased analysis of four distinct adipogenic cocktails to evaluate their effects on beige adipogenesis in inguinal stromal vascular frac

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12912031 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature