Development of a Flex-Seq SNP panel for raspberry (Rubus idaeus L.) and validation through linkage map construction and identification of QTL for several traits of agronomic importance to raspberry breeding.

ABSTRACT: High-throughput and reproducible genotyping platforms are critical for advancing genetic research and breeding in horticultural crops. Here, the development and validation of a custom single nucleotide polymorphism (SNP) panel using the Flex-Seq genotyping platform for red raspberry (Rubus idaeus L.) is described. SNPs were derived from existing linkage maps and RNA-seq data, resulting in a panel of 5,639 high-confidence, bi-allelic markers distributed across the seven chromosomes of the R. idaeus 'Malling Jewel' reference genome. The panel was used to genotype 457 red raspberry accessions including 161 individuals from a bi-parental mapping population (Paris×486), enabling the construction of high-density linkage maps and the identification of quantitative trait loci (QTL) for fruit size,

SUBMITTER: Davik J 

PROVIDER: S-EPMC12912553 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature