Development of serologic diagnostic test based on in silico predicted synthetic peptides for Brucella canis in dogs.

ABSTRACT: Serologic diagnosis of canine brucellosis caused by Brucella canis remains quite challenging since the currently available methods have considerable limitations, although the relevance and awareness about this zoonotic pathogen is increasing over the past few years. Therefore, the development of novel strategies is highly desirable. In this study, in silico analyses resulted in prediction of B. canis specific B cell epitopes, which were validated by synthesizing the corresponding peptides on a membrane followed by immunobloting with sera from dogs naturally infected with B. canis or uninfected controls as well as by excluding epitopes that cross reacted with sera from cattle and sheep infected with B. abortus and B. ovis, respectively. This approach resulted in the identification of 26 epi

SUBMITTER: Souza MFS 

PROVIDER: S-EPMC12912580 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature