96 sample parallel acoustic fragmentation for high throughput next generation sequencing library preparation.

ABSTRACT: Random, unbiased fragmentation of genomic DNA is necessary for next generation sequencing (NGS), yet the process of DNA fragmentation is still a bottleneck in NGS pipelines. A technology that increases the efficiency and consistency of this step will be highly desirable for both research laboratories and in clinical diagnostics. Previously, we reported the development of a novel cavitation enhancement reagent that dramatically decreases the time and acoustic energy required for genomic DNA fragmentation. The inclusion of this reagent in standard protocols facilitates highly efficient sonication enabling the use of widely available and inexpensive equipment, including water bath-based sonicators. Here, we report that cavitation enhancement facilitates parallel fragmentation of up to 96 samp

SUBMITTER: Mehrab-Mohseni M 

PROVIDER: S-EPMC12912608 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature