Introducing gold-standard essential gene datasets for Pseudomonas aeruginosa to enhance Tn-Seq analyses.

ABSTRACT: Transposon Sequencing (Tn-Seq) is a high-throughput technique that utilizes transposon mutant libraries to assess gene fitness or essentiality under specific conditions potentially identifying novel therapeutic targets. However, the diversity of statistical methods, bioinformatics tools, and parameters complicates the selection of the most appropriate and reliable analysis pipeline for a given dataset. A significant limitation of existing studies is the absence of a gold-standard set of essential genes (EGs) for evaluating the analysis process. Relying on the original study as a gold-standard is suboptimal, as these results may have been obtained using non-optimal tools. Here, we introduce reliable EG datasets for Pseudomonas aeruginosa to enhance Tn-Seq analyses. By utilizing literature d

SUBMITTER: Van Maele C 

PROVIDER: S-EPMC12912699 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature