<i>In situ</i> generation of dendritic cell vaccines in 3D printing scaffolds for cancer post-surgical therapy.

ABSTRACT: Dendritic cell (DC) vaccines have shown great promise in cancer management, while complex ex vivo cell culture limits their clinical applications. Here, we report an in situ-generated scaffold DC vaccine, comprising a simple 3D-printed gelatin methacryloyl, bone marrow mononuclear cells (BM-MNCs), tumor lysates and stimulating factors. Stimulating factors in this vaccine effectively initiated in situ differentiation of BM-MNCs into DCs, while personalized tumor lysates stimulated the maturation of differentiated DCs and enhanced their lymph node migration efficiency, thereby synergistically strengthening their antitumor effects. Moreover, the 3D hydrogel scaffold serves as an in situ cell culture matrix, promoting the long-term viability of encapsulated cells an

SUBMITTER: Chen L 

PROVIDER: S-EPMC12912718 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature