Development of a Duplex Digital PCR and Validation on eDNA Water Samples for Monitoring of the Asian Swamp Eel (<i>Monopterus albus</i>/<i>Javanensis</i>) and Bullseye Snakehead (<i>Channa aurolineata</i>/<i>Marulius</i>) in Florida, USA, Freshwater Ecosystems.

ABSTRACT: Invasive species are a significant threat to a variety of ecosystems in Florida, with freshwater habitats being of particular concern. Two nonnative species of fish with established populations that are priority organisms for monitoring and management programs are the bullseye snakehead (BS; Channa species complex) and Asian swamp eel (ASE; Monopterus species complex). Recent technological advances have seen the emergence of environmental DNA (eDNA) analysis as a useful tool for the detection and monitoring of target organisms and to assess removal efforts. In this study, a duplex digital polymerase chain reaction (dPCR) was developed, optimized, and validated on control samples and field samples from locations with documented occurrences of target organisms. The assay develo

SUBMITTER: Bloch M 

PROVIDER: S-EPMC12912945 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature