Orthologue inference-based enzyme mining for diversification of the anti-cancer evodiamine scaffold.

ABSTRACT: Selective functionalization is central to the structural and functional diversification of pharmacologically important alkaloids, yet the complexity of these scaffolds often hinders chemical modifications. Enzymes such as cytochrome P450 monooxygenases can overcome these challenges by catalyzing stereo- and regioselective transformations at typically inert C-H sites. Here, we present a targeted workflow for mining such enzymes by integrating orthologue-inference bioinformatics to leverage the increasingly available biological data. Comparative analysis of 15 alkaloid-producing plant species generated a focused library of 15 P450s, five of which exhibited activities toward alkaloid scaffolds. Four enzymes from Camptotheca acuminata and Tabernaemontana elegans selectively oxygenated two posi

SUBMITTER: Kwan BD 

PROVIDER: S-EPMC12913999 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature